Using Flame Photometer and Spectrophotometric Methods to Estimate the Minerals, Anti-oxidant Capacity, Total Phenol, and Total Carbohydrate of Nicotiana Glauca R.C. Graham. Plantago Major L. Sp. Pl and Phillyrea Latifolia L. Sp. Pl Plants

Khadijah Al-Awjali; Enaam Mohammed; Farag El-Mokasabi; Zuhir Ikraim; Hamad Hasan

doi:10.69667/lmj.261001

Authors

Khadijah Al-Awjali Botany Department, Faculty of Science, Derna University, Libya https://orcid.org/0000-0003-2824-5758
Enaam Mohammed Chemistry Department, Faculty of Education (Al-Marj), Benghazi University, Libya https://orcid.org/0000-0002-2782-8065
Farag El-Mokasabi Botany Department, Faculty of Science, Benghazi University, Libya https://orcid.org/0009-0006-0675-370X
Zuhir Ikraim Pharmacology and Toxicity Department, Faculty of Pharmacy, Omar Al-Muhtar University, Libya https://orcid.org/0000-0001-9373-721X
Hamad Hasan Chemistry Department, Faculty of Science, Omar Al-Mukhtar University, Libya https://orcid.org/0000-0002-6425-1883

DOI:

https://doi.org/10.69667/lmj.261001

Keywords:

Minerals, Carbohydrate, Antioxidant, Phenols, Plants, Flame, Spectrophotometer.

Abstract

The main aims of this study are to evaluate the concentrations of some minerals, potassium, sodium, and calcium, besides antioxidant, total phenols, and carbohydrate in leaf and stem samples of some plants, including Nicotiana glauca R.C. Graham, Plantago major L. Sp. Pl and Phillyrea latifolia L. Sp. Pl. selected from some eastern regions of Libya. The methods of the Flame photometer were used to estimate the contents of Minerals, and the Spectrophotometric method was used to estimate the concentration of carbohydrates, total phenols, and antioxidants. The results of this study showed that: all the studied samples containing higher values of potassium comparing with calcium and sodium contents, generally the contents of sodium were ranged from 0.708 to 1.45 ppm in leafs and from 1.68 to 9.88 ppm in stems, whereas the concentrations of potassium fluctuated in the ranges of 3.36 -37.56 ppm in leafs and from 36.96 to 75.76 ppm in stems. On the other side, the concentrations of calcium showed lower values compared with potassium and sodium, where the calcium contents ranged between 0.12 – 0.48 ppm in leaves and from 0.291 to 0.708 ppm in stems. The results also recorded that the contents of total phenols ranged between 195.49 – 345.33 ppm; a high content was observed in stems of Nicotiana glauca R.C. Graham. On the other side, the amounts of anti-oxidant were ranged from 9.124 ppm to 10.45 ppm in leaves and from 9.54 ppm to 10.008 ppm in Phillyrea latifolia L. Sp. Pl plant stems. Whereas the concentrations of total carbohydrate were fluctuated in the ranges from 0.054 to 0151 ppm in leaves and from 0.134 to 0.191 ppm in stems. Also, the phytochemical screening of the aqueous extracts of the selected leaf and stem samples showed the presence of different of natural product compounds.