Comparative Study of Hematological and Biochemical Parameters in Vaccinated versus Unvaccinated COVID-19 Patients in Al-Bieda City

Ali Shoaib; Ameena Husayn; Fayrouz Khaled; Majdi Abdulhamid

doi:10.69667/lmj.2517215

Authors

Ali Shoaib Department of Chemistry, Faculty of Arts and Science Alwahat, University of Benghazi, Libya https://orcid.org/0000-0002-3473-2651
Ameena Husayn The Higher Institute of Medical Sciences and Technologies, El-Beida, Libya https://orcid.org/0009-0008-2307-4797
Fayrouz Khaled Department of Chemistry, Faculty of Science, Omar Al-Mokhtar University, El-Beida, Libya https://orcid.org/0000-0002-3593-6277
Majdi Abdulhamid Department of Chemistry, Faculty of Science, Omar Al-Mokhtar University, El-Beida, Libya https://orcid.org/0000-0002-3364-9688

DOI:

https://doi.org/10.69667/lmj.2517215

Keywords:

COVID-19 vaccination, hematological parameters, biochemical markers, vaccinated vs. unvaccinated

Abstract

COVID-19 vaccination has played a crucial role in reducing the severity and spread of SARS-CoV-2 infection. However, questions remain regarding its systemic effects, particularly on hematological and biochemical parameters. This study aimed to assess the potential impact of COVID-19 vaccination on complete blood count (CBC) and selected plasma biochemical markers in patients by comparing vaccinated and unvaccinated individuals. A comparative analysis was conducted between vaccinated and unvaccinated COVID-19 patients. Hematological parameters assessed included red blood cell (RBC) count, hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell (WBC) count, and platelet count (PLT). Biochemical markers analyzed included plasma urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Statistical significance was considered at p < 0.05. The results showed no statistically significant differences (p > 0.05) in any of the CBC or plasma biochemistry parameters between vaccinated and unvaccinated groups. RBC count, Hb levels, HCT, and indices of red cell morphology (MCV, MCH, MCHC) remained comparable across groups, indicating that vaccination had no adverse effects on erythropoiesis or red cell function. WBC and platelet count also showed no significant changes, with values within physiological ranges. Similarly, urea and creatinine levels were slightly higher in the vaccinated group but not significantly different, suggesting preserved renal function. Liver enzyme levels (ALT, AST, and ALP) did not differ significantly, indicating no hepatic impairment post-vaccination

References

Alfituri A, Alasbily H, Alanieze F, Aboutrabah T. Complications following COVID-19 infection or vaccination in Libyan International Medical University students and their Families. Alq J Med App Sci. 2024;7(2):213-21.

Shama MA, Mehmood S, Zhang W. Pathological effects of SARS-CoV-2 associated with hematological abnormalities. Curr Issues Mol Biol. 2023;45(9):7161–7182.

Papadopoulou G, Manoloudi E, Repousi N, Skoura L, Hurst T, Karamitros T. Molecular and clinical prognostic biomarkers of COVID-19 severity and persistence. Pathogens. 2022;11(3):311.

Tanbakuchi D, Razavizadeh NT, Salari M, Farkhani EM, Shakeri MT, Tabatabaee SS, et al. Investigating the effectiveness of whole-virus, protein-based, and vector-based SARS-CoV-2 vaccines on the rates of COVID-19 infection, hospitalization, and mortality: a historical cohort study in Iran. BMC Infect Dis. 2025;25(1):44.

Chyad SA, Al Anbagi RA. Effect of COVID-19 and Its Vaccine: Hematological and Immunological Study of Recovered Individuals Based on Gender. Med J Babylon. 2024;21(2):375–382.

Butler B. COVID heritage imperatives as new pharmacologies of care. In: Rethinking Heritage in Precarious Times: Coloniality, Climate Change, and Covid-19. 2023.

Zhang Z, Gao S, Dong M, Luo J, Xu C, Wen W, et al. Relationship between red blood cell indices (MCV, MCH, and MCHC) and major adverse cardiovascular events in anemic and nonanemic patients with acute coronary syndrome. Dis Markers. 2022;2022(1):2193343.

Sing CW, Tang CTL, Chui CSL, Fan M, Lai FTT, Li X, et al. COVID‐19 vaccines and risks of hematological abnormalities: nested case–control and self‐controlled case series study. Am J Hematol. 2022;97(4):470–478.

Klok FA, Pai M, Huisman MV, Makris M. Vaccine-induced immune thrombotic thrombocytopenia. Lancet Haematol. 2022;9(1):e73–e80.

Mir SM, Tahamtan A, Nikoo HR, Arabi MS, Moradi AW, Ardakanian S, Tabarraei A. Evaluation of biochemical characteristics of 183 COVID-19 patients: a retrospective study. Gene Rep. 2022;26:101448.

Gounden V, Bhatt H, Jialal I. Renal function tests. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024.

Kamal A. Estimation of blood urea (BUN) and serum creatinine level in patients of renal disorder. Indian J Fundam Appl Life Sci. 2014;4(4):199–202.

Rammohan R, Joy M, Saggar T, Magam SG, Sinha A, Natt D, et al. Investigating the impact of COVID-19 vaccines on liver function: insights from a single-institute study. Cureus. 2023;15(3).

Abdelmola HJ, Khaled FA. Comparison of height, weight, vitamin D, calcium and parathyroid hormone between males and females at the Tobruk University. In: Sebha University Conference Proceedings. 2024. p. 281–290.